簡要描述：BD Purified Rat Anti-Mouse CD16/CD32 553141，BD 553141 Pharmingen 純化大鼠抗小鼠 CD16/CD32 抗體，常用于流式細胞術和免疫組織化學等實驗中，作為 BD Fc 阻滯劑。該抗體通過阻斷小鼠 Fc 受體（CD16/CD32）與 Fc 區(qū)域結合，防止非特異性結合，從而提高下游實驗中抗體標記的特異性和準確性。
產品型號：- 廠商性質：代理商
- 更新時間：2025-06-10
- 訪 問 量：455
產品介紹
| 品牌 | BD/美國 | 貨號 | 553141 |
|---|---|---|---|
| 規(guī)格 | 0.1mg | 供貨周期 | 兩周 |
BD Purified Rat Anti-Mouse CD16/CD32 553141
純化大鼠抗小鼠CD16/CD32小鼠Fc阻滯劑
Brand:BD Pharmingen™
Alternative Name:FcγRIII/FcγRII; Fcgr3/Fcgr2
Reactivity:Mouse (QC Testing)
Isotype:Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
Immunogen:Mouse BALB/c Macrophage J774
Application:Blocking, Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunoprecipitation (Reported)
Concentration:0.5 mg/ml
Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status:RUO
準備和存儲
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
推薦的實驗流程
To specifically stain cells bearing FcγII and FcγIII receptors for flow cytometric analysis: Incubate cell suspension with this antibody (≤ 1 μg/million cells) followed by an appropriate fluorochrome-conjugated second-step reagent.
To reduce Fc receptor-mediated binding by antibodies of interest or Fc receptor-mediated binding by PE-CY5 tandem dye conjugates to FcγII and FcγIII receptor-bearing mouse cells for flow cytometric analysis:
1. Preincubate cell suspension with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (eg, ≤ 1 μg/million cells in 100 μl) at 4?C for 5 minutes.
2. Add antibody of interest directly to preincubated cells in the presence of Mouse BD Fc Block™ (ie, Mouse BD Fc Block™ need not be washed off before staining cells).
3. If anti-Ig second-step is necessary, a reagent must be chosen which will not bind to Mouse BD Fc Block™ (eg, rat IgG2b, κ).
BD Purified Rat Anti-Mouse CD16/CD32 553141
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